Significant Differences in Host-Pathogen Interactions Between Murine and Human Whole Blood

Murine infection models are widely used to study systemic candidiasis caused by C. albicans . Whole-blood can help elucidate host-pathogens interactions and have been for several Candida species in human blood. We adapted the whole-blood model murine Unlike blood, blood was unable reduce fungal burden more substantial filamentation of observed. This coincided with less association leukocytes, especially neutrophils. The lower neutrophil number only partially explains insufficient control, as spiking neutrophils had limited effects on killing. Furthermore, increased survival is not mediated enhanced filamentation, a filament-deficient mutant likewise eliminated. also observed host-dependent differences interaction platelets , showing platelet aggregation, adhesion activation For opsonization shown decrease suggesting that complement factors interfere fungus-to-platelet binding. Our results reveal between infected thereby demonstrate limitations translatability this ex vivo hosts.